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gateway 5 multisite cloning kit  (Thermo Fisher)


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    Thermo Fisher gateway 5 multisite cloning kit
    Gateway 5 Multisite Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gateway+5+multisite+cloning+kit/ppr0951772-199-4-12
    Average 90 stars, based on 1 article reviews
    gateway 5 multisite cloning kit - by Bioz Stars, 2026-09
    90/100 stars

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    Cloning:

    Article Title: Buffering of nuclear membrane tension and mechanotransduction by the endoplasmic reticulum revealed by quantitative ALPIN imaging
    Article Snippet: Fertilized casper zebrafish 3 embryos were collected and injected at the one-cell stage into the cytoplasm with a Nanoject IITM 4 microinjector (Drummond Scientific, Broomball, PA). .. Plasmids were assembled using Gateway 5 multisite cloning kit using LR ClonaseTM (Invitrogen; C12537-023) and multicloning sites between 6 the gateway att sites flanking cassette of p5E (hsp70i), pME(cPla2-mKate2) and p3E(P2A-eGFP-7 KDEL) plasmids that were recombined into Tol2kit Destination pDESTcrybb1 for generating 8 Tg(hsp70i:cPla2-mKate2-P2A-eGFP-KDEL). .. The middle entry pME vector was assembled from 9 cytosolic phospholipase A2 (cPla2, Ensembl: ENSDARG00000024546), open reading frame 10 amplified by PCR and ligated into pDONOR221 containing pME gateway compatible att sites and 11 subsequently fused in-frame to mKate2 (Evrogen) as a C-terminus fluorescent tag with a 15 12 amino-acid GS-enriched linker.



    Similar Products

    gateway 5 multisite cloning kit  (Thermo Fisher)
    90
    Thermo Fisher gateway 5 multisite cloning kit
    Gateway 5 Multisite Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gateway+5+multisite+cloning+kit/ppr0951772-199-4-12
    Average 90 stars, based on 1 article reviews
    gateway 5 multisite cloning kit - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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